You are tasked with making a series of Furosemide calibration standards for analysis by HPLC with fluorescence detection.
(a) Given Furosemide has a molecular weight of 330.7 g/mol, what weight of furosemide would you need to make a stock solution of 0.001 M (10-3 M) concentration ?
(b) How would you then make up a series of standards from this stock solution, of concentrations 10-4, 10-5, 10-6, 10-7 and 10-8 M Furosemide?