The following figure shows the general steps that occur when a researcher uses the CRISPR-Cas9 system to modify a protein-encoding gene in a eukaryotic cell with the goal of modifying the protein product. Drag the descriptions of the steps to their appropriate locations on the figure.
1. Create plasmid expression vectors with genes encoding Cas9 and sgRNA.
2. Introduce the vectors into a eukaryotic cell.
3. The gene expression machinery of the cell produces the Cas9 protein and the sgRNA.
4. The Cas9 protien, guided by sgRNA, binds to its target sequence in the cell's DNA.
5. The Cas9 protien cleaves the target sequence.
6. During the process of DNA break repair, the target sequence may be modified.
7. The modified DNA sequence is transcribed into RNA.
8. the RNA is translated into a modified protien.