Shown below is a DNA sequence before exposure to Cla This sequence is labeled "No Fragmentation" and has the recognition site of Cla1 in bold text. Below this is the sequence after exposure to Cla1 showing fragment 1 (in blue) and fragment 2 (in red).
No Fragmentation
5’ – GAGACCATCGATACCGGC – 3‘
3’ – CTCTGGTAGCTATGGCCG – 5’
Fragment 1 Fragment 2
5’ – GAGACCAT CGATACCGGC – 3‘
3’ – CTCTGGTAGC TATGGCCG – 5’
Scientist have discovered significantly more restriction endonucleases than what is shown in your investigative manual. BamH1, for instance, is a commonly used restriction endonuclease with the recognition and site and cleavage pattern:
BamH1 Recognition site
5’ – GGATCC – 3‘
BamH1 Cleavage pattern
5’ – G/GATCC – 3‘
5’ – CCTAG/G – 3‘
In consideration of this produce the fragments of the following DNA sequence if it were exposed to BamHI:
No Fragmentation
5’ – GAGACCGGATCCACCGGC – 3‘
3’ – CTCTGGCCTAGGTGGCCG – 5’
Fragment 1 Fragment 2