In the third step of the cloning procedure,all of the plasmids that were created in the second step are incubated with bacteria. The bacteria do not have an ampR gene on their own chromosome.
As shown above at least four classes of bacteria result. Notice that only one class contains the vgp gene.
To help sort out those bacteria that have the vgp gene, scientists first attempt to grow the bacteria both in a medium with ampicillin and in a medium without ampicillin. Demonstrate your understanding of this step by sorting the four bacterial classes based on where they will grow.
Sort the bacterial class into the appropriate category.
becaterial class: -no plasmid, -nonerecombinant plasmid, -recombinant plasmid but no vgp gene, -recombiant plasmid with vgp gene
will ONLY grow in medium without ampicillin:
will ONLY grow in medium containing ampicillin:
will grow in both media: