The kinetics of an enzyme are measured as a function of substrate concentration in the presence and absence of 100 µM inhibitor.

[S] (microMolar): 3, 5, 10, 30, 90

Velocity (micromol/min) No Inhibitor: 10.4, 14.5, 22.5, 33.8, 40.5

WITH Inhibtor:2.1, 2.9,4.5, 6.8,8.1

If [S]=30 μM, what fraction of the enzyme molecules has a bound substrate in the presence of 100 μM100 μM inhibitor?

fES= _____