You are given a protein solution with a concentration of 0.25 mg/ml.
a) You need 100 μg of protein for an experiment. What volume of the protein solution do you need?
b)Suppose you want to prepare a solution containing 200 μg of the protein at a concentration of 1 mg/ml. To do this you will first freeze-dry enough protein solution to get 200 μg of the protein. How much solution will you need to freeze-dry? How much water will be added to the dried protein to get the desired 1 mg/ml concentration?
c) If the protein has a M.W. = 22,000, express its initial concentration in moles/liter, in μ moles/ml and in μ moles/μl. If you need 2 μ moles for a reaction, what volume do you need?
d) Suppose you want 1 ml of a 10 μg/ml solution. How much water and protein stock must you add to get this?
e) Suppose you want 100 mu l of a 0.1 μg/mu l solution. How much water and protein stock must you add to get this?