An Hfr strain of E. coli with the genotype gly+aziR StrS is mated with an F-strain of E. coli of genotype gly- aziSStrR. Gly refers to the amino acid glycine, azi issodium azide, and Str is antibiotic streptomycin, where S and R aresensitive and resistant, respectively. Conjugation occurs and theprogeny are screened on a selective medium to detect recombinants.If you wanted to select for the F- recombinant genotypegly+ aziR StrR, you should use aminimal medium containing:
a. sodium azide
b. streptomycin
c. glycine
d. glycine and streptomycin
e. streptomycin and sodium azide