Malate Dehydrogenase catalyzes the following reaction in liver mitochondria:
Malate + NAD --> Oxaloacetate + NADH + H
Consider the free energy changes for the following reactions:
Malate --> Oxaloacetate + 2e- ∆Gᵒ¹ = 249 kJ/mol
NADH + H⁺ --> NAD⁺ + 2H⁺ + 2e- ∆Gᵒ¹ = 220 kJ/mol
What is the ∆Gᵒ¹ for the following coupled reaction:
Measured malate in mitochondria is 0.20 mM, NAD+ 1.0 mM and NADH 0.01 mM. oxaloacetate = 1x10-7M (ignore the H+ for this problem)...
What is the keq for the reaction?
