Based on the provided data, what conclusion can be drawn about the impact of heating, cooling, and the presence of a chaperone on the activity and solubility of the Twinkle protein?

A) Heating alone significantly increases the activity of Twinkle.
B) Cooling alone has no effect on the activity of Twinkle.
C) The presence of a chaperone during cooling rescues the activity of Twinkle.
D) Solubility is positively correlated with protein activity in all conditions.