Which was not an experiment performed to determine if the "High DNA concentration effect" was a valid hypothesis?
a) Perform the TaqMan reactions without adding the Taq polymerase and then look at the ΔRQ values.
b) Perform the TaqMan reactions and determine the ΔRQ values over time on a per cycle basis.
c) Perform the TaqMan reactions for 60 cycles to see if the ΔRQ values declined.
d) None of the above were experiments performed.