What wavelength settings would you set on the excitation and emission monochromators to measure:
i) Excitation spectrum
ii) Fluorescence emission spectrum
iii) Phosphorescence emission spectrum
a) Excitation spectrum: Fixed wavelength; Fluorescence emission spectrum: Scanning from shorter to longer wavelengths; Phosphorescence emission spectrum: Fixed wavelength.
b) Excitation spectrum: Scanning from shorter to longer wavelengths; Fluorescence emission spectrum: Scanning from shorter to longer wavelengths; Phosphorescence emission spectrum: Scanning from longer to shorter wavelengths.
c) Excitation spectrum: Scanning from shorter to longer wavelengths; Fluorescence emission spectrum: Fixed wavelength; Phosphorescence emission spectrum: Scanning from longer to shorter wavelengths.
d) Excitation spectrum: Fixed wavelength; Fluorescence emission spectrum: Fixed wavelength; Phosphorescence emission spectrum: Scanning from longer to shorter wavelengths.