Albina now wants to screen her isolated colonies to determine whether they have resistance geneA. She picks 5 total colonies, lyses them at 95ºC and decides to set up a PCR reaction using appropriate primers. These primers should give a 4kb amplicon if resistance gene A is present. She has primer stocks of 100uM and a diluted version at 5uM. She also has a bacteria with plasmid that she knows contains resistance geneA. How should she set up her PCR? Describe how to make the master mix. Template DNA: 1.0 uL Primer 1 (0.25uM): Primer 2 (0.25uM): 2X PCR master mix: Water: Total 50.0 uL