You ask a new trainee to perform a fluorescent immunostain on the MIT cell line using the anti-FETT GFP-tagged antibody. The trainee accidentally makes an error while performing the immunostain and this impacts the results.
i) Compare the expected experimental results to the actual results obtained by the trainee and describe any differences. (1 mark) (1-2 sentences)
ii) Which step in the immunostaining protocol did the trainee likely omit or perform incorrectly? Explain how this error resulted in the trainee images shown above. (2 marks) (2-3 sentences)