You are preparing a viable cell count of bacteria. The original broth has an unknown concentration of bacteria. You dilute the culture 1/10,1/10,1/10, and 1/10 and plate 0.1 mL from the last tube onto a dish with nutrient agar. The following day you count the number of colonies on the plate with the following results: 1400 cells
a.) Draw a diagram of this procedure.
b.) What was the concentration of bacteria in the original broth per mL ?
c.) What was the concentration of bacteria per mL in diluted tubes 1,2,3, and 4?
d.) If the original broth contains 140 mL, how many cells are present before transfer?