You have been given a project to study the newly discovered aromatase-kinase H (AKH), a ~41 kDa protein. A colleague has isolated a ~1400-bp Xho I restriction fragment of human CDNA that molecular probes indicate contains the akh gene.
Your assignment is to clone the gene to produce sufficient AKH for further study. Insert the restriction fragment containing the akh gene into the PGLO Induce coli bacterial cells to take up the recombinant PGLO plasmid.
• Verify the presence of the PGLO plasmid carrying the akh gene in the bacteria by RFLP & PCR. Verify the expression of the akh gene in the bacteria by purifying the AKH protein from transformed bacterial cultures.