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In sexual reproduction, two different strains of hyphae called plus and minus strains fuse nuclei and produce a zygote. The third option in this question is the correct option. It is not as easy as it sounds in this question, but it is far more complicated than this.

In sexual reproduction, two different strains of hyphae called PLUS and MINUS strains fuse nuclei and produce a zygote.  

Sex chromosomes determine the sex of an organism. The different sexes are listed as either the plus strain or the minus strain. Both the strains fuse their nuclei and produce a zygote.                                                  

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