Answer: The correct answer is 1) biological species are defined by reproductive isolation and 3) biological species are the largest unit of population in which successful reproduction is possible.
According to biological species concept, two organisms belong to same species if they are capable of interbreeding and producing viable fertile offsprings.
This means that group of these members belonging to the same species is reproductively isolated from other group of species. Thus, according to this concept, successful reproduction is possible.
Therefore, option 1) and 3) is the right answer.