During transcription in eukaryotes, a type of RNA polymerase called RNA polymerase II moves along the template strand of the DNA in the 3'→5' direction. However, for any given gene, either strand of the double-stranded DNA may function as the template strand.

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Answer:

The answer is yes, either strand of DNA could function as template.

Explanation:

The structure of DNA involves two strands that are disposed of in a helicoidal shape. However, what makes DNA the molecule of genetic heritage is the correspondency of the nitrogen bases. There are 4 bases (Adenine, Guanine, Cytosine, and Thymine) and each one corresponds to another, in every case Adenine and Guanine bind together and Cytosine and Thymine also bind together. Therefore, the information contained in one strand is the same contained in the other, because the strands are always assembly in the same way but with different bases (corresponding one another).

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