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During electrophoresis, DNA molecules can easily be separated according to size because all DNA molecules have the same charge–mass ratio and the same shape (long rod). Would you expect RNA molecules to behave in the same manner as DNA during electrophoresis? Why or why not? Klug, William S.. Essentials of Genetics (p. 181). Pearson Education. Kindle Edition.

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Answer:

To separate DNA using agarose gel electrophoresis, the DNA is loaded into pre-cast wells in the gel and a current applied.

Explanation:

The phosphate backbone of the DNA (and RNA) molecule is negatively charged, therefore when placed in an electric field, DNA fragments will migrate to the positively charged anode.

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