Answer:
3. The ETC cannot establish a proton gradient to drive chemiosmotic production of ATP
Explanation:
Reduction of FAD+ and NAD+ during glycolysis and Kreb's cycle produces FADH2 and NADH respectively which in turn give their electrons to the molecular oxygen via electron transport chain (ETC). This electron transfer is accompanied by the pumping of protons towards the inter-membrane space resulting in the generation of an electrochemical gradient.
The energy of the electrochemical gradient is harnessed to drive ATP synthesis. The process is called chemiosmotic ATP synthesis. The mentioned insecticide would not allow the FADH2 and NADH to reduce oxygen and no electrochemical gradient is developed to drive the chemiosmotic production of ATP.