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1) If you needed to make 250. mL of a 1x TBE buffer, how much of each of the following would you use: solid Tris base (121.1 g/mol); solid boric acid (61.8 g/mol); liquid stock solution of 0.50 M EDTA?

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Answer:

for 1 Litre(1000 ml) of  1x TBE

10.8 g Tris

5.5 g Boric acid

4 ml 0.5 M EDTA (pH 8.0)

900 ml distilled water

Adjust volume to 1 Liter.

so for 250 ml divide everything by 4

2.7 g Tris

1.37 g Boric acid

1 ml 0.5 M EDTA (pH 8.0)

225 ml distilled water

Adjust volume to 250 Ml

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