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Transgenic models can be generated using CRISPR/Cas9 to introduce cancer-related mutations into cells within an organism. Which of the following approaches can produce a transgenic model? (Mark all that apply)

(A) Patient derived xenografts (PDFs)
(B) Viral delivery
(C) Lipid nanoparticles
(D) Knockin a wildtype genetic locus
(E) Direct nucleic acid (plasmid) injection

Respuesta :

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Answer: A, B, C, D, E

Explanation:

All the methods listed in the options above have been employed in gene transfer and the generation of transgenic model. Most of the options have been used to produce transgenic rat models for the study of human diseases.

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