A specific steroid has a λmax of 257 nm and a molar absorptivity ε of 11 , 500 L mol − 1 cm − 1 . What is the concentration of the compound in a solution whose absorbance at 257 nm is A = 0.090 with a sample pathlength of 1.00 cm ?

Respuesta :

Answer:

[tex]7.83\times 10^{-6} M[/tex] is the concentration of the compound in a solution.

Explanation:

Using Beer-Lambert's law :

Formula used :

[tex]A=\epsilon \times C\times l[/tex]

Where:

A = absorbance of solution

C = concentration of solution

l = path length

[tex]\epsilon[/tex] = molar absorptivity coefficient

We have:

C = ? , l = 1.00 cm, A = 0.090

[tex]\epsilon = 11,500 L/(mol cm)[/tex]

[tex]C=\frac{A}{\epsilon l}=\frac{0.090}{11,500 L/(mol cm)\times 1.00 cm}[/tex]

[tex]C=7.83\times 10^{-6} M[/tex]

[tex]7.83\times 10^{-6} M[/tex] is the concentration of the compound in a solution.

The concentration of the compound in a solution whose absorbance at 257 nm is [tex]7.83*10^6 M[/tex].

Beer-Lambert's law:

It states that there is a linear relationship between the concentration and the absorbance of the solution. It is given by:

[tex]A=E*l*c[/tex]

where:

A = absorbance of solution

c = concentration of solution

l = path length

E = molar absorptivity coefficient

Given:

l = 1.00 cm

A = 0.090

E= 11,500L/mol/cm

To find:

C = ?

Substituting the values in the above formula:

[tex]A=E*l*c\\\\c=\frac{A}{E*l}\\\\ c=\frac{0.090}{11,500L/(molcm)*1.00cm} \\\\c=7.83*10^6M[/tex]

Thus, the concentration of the compound in a solution is [tex]7.83*10^6 M[/tex].

Find more information about Beer-Lambert's Law here:

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