the functioning of DNA and RNA are affected by various enzymes. Sometimes, environmental factors can influence how these enzymes work. Which of these are factors that can change enzyme functionality?

The concentration of an enzyme can be hugely important for its functionality, with increasing concentration, the enzyme can increase the reaction rate. This means that if the concentration of an enzyme is low, it might not be able to perform its functions optimally as the amount of substrate could be too high relative to the amount of enzyme.

The location of an enzyme is hugely important. It has to be in the correct area of the cell in order to contact its substrate. In this case, the substrates are DNA/RNA, so location on the molecule is important. For example, RNA polymerase is needed to transcribe RNA from DNA, but it should be at the correct location (at the correct gene) in order to perform its function.

Temperature and pH are important environmental conditions that contribute to enzyme function. Enzymes will have a range of temperatures and pH for which it is functional, and within that, a more specific optimal temperature and pH at which it performs its best. Outside this range, the enzyme will not perform its function properly. At very high temperatures and at very low/high pH, the enzyme could even become denatured and destroyed.