Which of the following is not an assumption that is made when applying Michaelis-Menten kinetics to an enzyme? Choose one:

A. The rate constant of E+Pre-associating to form the ES complex must be considered

B. The reaction must be considered early, before any appreciable amount of product has been generated.

C. The product release is a rapid step in the process

D. The concentration of ES is relatively constant after the initial reaction time.