Polymerase chain reaction (PCR) is a common laboratory technique used to make many copies of a particular region of DNA. PCR is usually used to create enough of the target DNA. DNA replication in organisms requires a DNA polymerase to create new strands of DNA from existing templates and this is needed in PCR. How does PCR separate the DNA strands to prepare for DNA replication?
The PCR machine consist of a thermal cycle that increases and decreases the temperature of the sample. When the temperature is increased, the double-stranded DNA template mixture is denatured or separated into single strands.
