Answer:
50 ml (5x TBE) + 540 ml (water)
Explanation:
To prepare 0.5x TBE solution from 5x TBE solution we need to use the following dilution formula:
C1 x V1 = C2 x V2, where:
- C1, V1 = Concentration/amount (start), and Volume (start)
- C2, V2 = Concentration/amount (final), and Volume (final)
* So when we applied this formula it will be:
5 x V1 = 0.5 x 500
V1= 50ml
- To prepare 0.5x we will take 50ml from 5x and completed with 450ml water and the final volume will going to be 500ml.
