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This group of questions consists of five lettered headings followed by a list of phrases or sentences. For each phrase or sentence, select the one heading to which it is most closely related. Each heading may be used once, more than once, or not at all. This group of questions refers to the following enzymes. (A) DNA ligase (B) DNA polymerase (C) RNA polymerase (D) Restriction enzyme (E) Reverse transcriptase Enzyme used during replication to attach Okazaki fragments to each other

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samueladesida43

Answer:

A (DNA LIGASE)

Explanation:

Replication of DNA cannot occur without certain enzymes with varying functions. After unwinding of the DNA double-strand by helicase enzyme, DNA polymerase attaches itself and starts adding nucleotides, however, DNA polymerase can only add nucleotides in the 5'-3' direction. Since the separated strands run in an anti-parallel manner i.e. one runs from 5'-3' while the other from 3'-5'.

Nucleotides are continuously added to the leading strand (runs from 5'-3') since that is the normal direction of synthesis by DNA polymerase but because of the 3'-5' direction of the lagging strand, nucleotides are added in small fragments called OKAZAKI FRAGMENTS. As the polymerase acts on the leading strand, these okazaki fragments are made on the opposite strand (lagging strand).

An enzyme called DNA LIGASE is responsible for attaching from end to end, the small okazaki fragments on the lagging strand to one another.