Answer:
0.2 and 0.1
Explanation:
Thin Layer Chromatography (TLC) is a type of separation technique which involves a stationary phase (an adsorbent medium) and a mobile phase (a solvent medium), and is used to separate mixtures of non-volatile compounds based on their relative attractions to either phases, which is determined by their polarity.
The more a compound binds to the adsorbent medium, the slower it moves up the TLC plate. Compounds that are polar tend to move up the TLC plate slower than non-polar compounds, resulting in a lower Rf value for polar compounds and a higher Rf value for non-polar compounds.
Rf (Retention factor) = distance moved by compound/solute
distance moved by the solvent front
Rf values of 0.3 and 0.1 gives a difference of 0.2
Rf values of 0.8 and 0.6 gives a difference of 0.2
Rf values of 0.5 and 0.8 gives a difference of 0.3
Rf values of 0.2 and 0.1 gives a difference of 0.1, therefore the smallest separation between compounds is the one with Rf values of 0.2 and 0.1.
