Answer:
5' RG GWCCY 3'
3' YCCWG GR 5'
Explanation:
The enzyme PpuMI is a restriction endonuclease enzyme, it has a specific recognition site where it cut the DNA. The source of the enzyme is from an E. coli strain that carries the PpuMI gene from Pseudomonas putida (R. Morgan).
The enzyme PpuMI recognizes specific sequence with palindrome arrangement. It target the sequence 5' RGGWCCY 3'
target Sequence: 5' RGGWCCY 3'
3' YCCWGGR 5'
The enzyme cleavage point is at:
5' RG^GWCCY 3'
3' YCCWG^GR 5'
The product of the cleavage will give a sticky end Cleavage:
5' RG GWCCY 3'
3' YCCWG GR 5'
Note: R stands for purines (adenine and guanine). Y stands for pyrimidines (cytosine, thymine, and uracil). And W represents adenine or thymine.
