In E. coli, there is a mutation in a gene called dnaB that alters the helicase that normally acts at the origin. Which of the following would you expect as a result of this mutation

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Answer:

The question lacks options, the options are:

A) No proofreading will occur. B)The DNA will supercoil. C)Replication will occur via RNA polymerase alone.

D)No replication fork will be formed.

E)Replication will require a DNA template from another source.

The answer is D

Explanation:

Prior to division of any cell, its genetic material needs to be replicated. Ideally, in a bacterial cell, this replication starts at a particular nucleotide sequence called ORIGIN OF REPLICATION. An enzyme called DNA HELICASE then binds to the origin of replication and breaks the hydrogen bonds between the double-stranded DNA molecule to form a Y-shaped Replication fork, which serves as a template for DNA polymerase III to bind and begin to add nucleotides.

However, if a mutation occurs at a certain gene dNAb and alters the action of HELICASE enzyme. Thus, it prevents the helicase from separating the double-strands of the DNA molecule containing the gene. Hence, no replication fork will be formed and subsequently no template for DNA polymerase's action.

Helicase proteins unravel and unzip its a double DNA helix, producing a replication fork, therefore, enabling each DNA strand to replicate. Those proteins bind to Stranded DNA or unzip it all through the lengths of the DNA, allowing for effective replicating.

  • When the enzymes were altered or nonfunctional, the procedure of unzipping the strands would be impeded, which would eventually disrupt the regeneration process.  
  • When the enzyme was altered, the DNA strands may separate at the origin, but reproduction would not proceed.  
  • The strands will not be unzipped ahead of such origin, and also no free strand would be available for the replication machinery.

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