Site-directed mutagenesis is a method in biochemistry/ molecular biology that introduces small changes into the DNA sequence of a gene. This change alters the amino acid sequence of a protein and can alter the function of a protein. What changes are typically inserted into the DNA sequence?
Site-directed mutagenesis alters the amino acid sequence by introducing changes in the base sequence of DNA. This is done by either by substituting a single base, multiple bases and inserting or deleting a base.