Since, the experiment is not given but on the basis of the mechanism of genetic transfer this question can be answered.
Answer:
The mechanism of gene transfer is important and beneficial for bacteria in the adverse environment conditions. The different mechanism of gene transfer are conjugation, transformation and transduction.
The ampicillin resistance gene is present on the plasmid DNA and not on the chromosomal DNA. Only conjugation is the mechanism in which the plasmid DNA is transferred from one bacteria to another bacteria. The ampicillin resistance gene is transferred from Strain II to strain I means from the bacteria that has the ampicillin resistance gene to the bacteria that has no ampicillin resistance gene in it.