Fill in the blanks to describe the process of making recombinant DNA. Remove desired ___ from the donor cell. Remove bacteria . Insert desired gene into plasmid. Return plasmid to _. Gene will now be transcribed and translated into a ____ product.

Recombinant DNA Technology is a techniques used to produce, transfer and express a desired gene in an expression system that produces the protein product in bulk. Recombinant DNA technology involves the following steps:

Selecting the desired gene or gene of interest (GOI).
Cutting/extracting the gene of interest from the organism by using restriction endocucleases i.e. enzymes that cut DNA at specific sites and produce "sticking ends" that enable the gene to reattach to a plasmid.
Extracting a plasmid from a bacterium and cut it with the same restriction enzyme.
Reinsert the plasmid vector (recombinant DNA) into an expression system (bacteria).
The bacteria will translate the gene into the desired protein product.

Fill in the blanks to describe the process of making recombinant DNA. Remove desired _______ from the donor cell. Remove bacteria ________. Insert desired gene into plasmid. Return plasmid to _________. Gene will now be transcribed and translated into a ________ product.

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Fill in the blanks to describe the process of making recombinant DNA. Remove desired ___ from the donor cell. Remove bacteria . Insert desired gene into plasmid. Return plasmid to _. Gene will now be transcribed and translated into a ____ product.