Referring to the triplicate Bradford data for the E3 fraction, what sample volumes did you use in the assays and what microplate absorbance values did you record?Show how you calculated the total amount of protein and the standard deviation for the E3 fraction that you plotted on your "combined elution/activity profile."

Please give a detailed answer

There are 12 fractions performed in triplicate Bradford data.1 ml of Bradford reagent and 50μl of protein is taken and mixed in a test tube for total volume of 1.05ml.The value taken of Bradford assays by microplate agent are compared through standard curve. This standard curve is made by using specific amounts of protein within each Bradford and then calculated by linear equation of standard curve.

Linear equation for standard curve is Y=0.0245 x + 0.03605

For the 1st trial of E3 :

it can be written as 0.511 = 0.0245 x + 0.3605 + x→ = 6.14μg

For the 2nd trial of E3 :

it can be written as 0.489 = 0.0245 x + 0.3605 + x→ = 5.24μg

For the 3rd trial of E3 :

it can be written as 0.478 = 0.0245 x + 0.3605 + x→ = 4.80μg

standard derivation = √∑ | x - average|²

n-1

by putting values in derivation, we will get 0.683

and

Standard derivation = √?