If a fluorescent green color is observed in the E. coli colonies then a new question might well be raised, "What are the two possible sources of fluorescence within the colonies when exposed to UV light?" to UV light?io Explain 1. Recall what you observed when you shined the UV light onto a sample of original Cvelotipl sony planeel cal biotechsclegtde/를 mia contains green fluorescent protein Thevebe P lo plosmid is ueedl as avecter In biotech nalaq lsnie contains a reen fluorescent Brotei protein hen uV aht Shine oul Which of the two possible sources of the fluorescence can now be eliminated? nce, 2. 3. What does this observation indicate about the source of the fluorescence? Describe the evidence that indicates whether your attempt at performing a genetic transformation was successful or not successful

1. When we shined the UV light onto the negative pGLO plasmid DNA we did not see anything important and the colonies were unaffected. When we pour UV light on the positive pGLO plasmid DNA then results were different we saw the colonies in the plates fluoresce a green color. This shows that protein is present in the plate and it fluorescing due to it.

2. Two possible sources of the fluorescence which can now be eliminated. These sources include the pGLO plasmid DNA and the original bacterial cells.

3. The source of fluorescence is from the plasmid. The pGLO plasmid has a green fluorescent protein that allows the transformed E.coli to glow under UV light.

4. Successful experiments shown by presence of colonies within the (+) LB/amp/ara plate. The (+) LB/amp/ara plate also fluoresced green under the presence of UV light. And on the other hand the (-) LB/amp had no sign of colonies, which shows the success of the experiment. One factor of the experiment that did not help the success of the experiment was the presence of colonies among the (-) LB/Amp plate and the glowing of the colonies under the UV light. In addition, the (+) LB/Amp plate showed no sign of colonies lthere can also be some errors in the experiments which lowers the success rate.

Explanation:

kasliwalalvi24 kasliwalalvi24 · Answer 2 · 2021-12-02T13:31:12+01:00

1. The negative pGLO vector plasmid when exposed to the UV light no important colonies were observed. The positive pGLO plasmid when exposed to the UV light resulted in the colonies.

The colonies were identified by the fluorescence green color due to the presence of protein in the plate.

2. The sources of fluorescence can be pGLO plasmid, bacterial cells, and DNA. These sources can now be eliminated.

3. The pGLO plasmid is a source of fluorescence. The plasmid consists of the green fluorescent protein, which allows the E.coli transformed to glow when exposed to UV light.

4. The colonies with positive plasmid were found on the plates of LB, amp, and ara. These plates showed green fluorescence in the presence of UV light.

The negative plasmid did not form any colonies on the plates of LB, amp, and ara. It shows the successful experiment.

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