Answer:
This is an unexpected result. Absence of primers means that DNA polymerase cannot bind with the template DNA and allow its amplification. If Phil sees a positive band, it should be because of contamination of primers (same set of primer or other) with DNTPs, water, MgCl2, or PCR buffer. This is the reason that a negative run for PCR is always recommended.
This is sudden as it is an unexpected result after setting up a PCR reaction Phil noticed that he'd forgotten to put primers into the mix. When he runs a gel of the PCR reaction after the reaction is finished he sees a positive result.
Absence of primers manner that DNA polymerase can not bind with the template DNA and permit its amplification. If Phil sees a high-quality band, it ought to be due to infection of primers (identical set of primer or other) with dNTPs, water, MgCl2, or PCR buffer. This is the motive that a terrible run for PCR is continually recommended.
PCR stands for a polymerase chain reaction. This is a test to identify genetic material from a particular organism. B Virus to detect. If the virus is present at the time of the test, the test will detect the presence of the virus. This test can also detect virus fragments even when they are no longer infected.
Hence concluded that it is an unexpected result.
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