The question is inappropriate which should be as the following:
A.) What is the dilution factor after Step 1? (2 points)
B.) What is the final/total dilution factor at the end of Step 2 from the starting stock?
Answer:
1. Df- 1/4 r 4 fold or 4 times
2. Final dilution factor- 12 times
Explanation:
A serial dilution refers to the reduction in the concentration of the solute in a solution in which the solution is diluted with the same dilution factor.
In the given question,
The dilution factor will be calculated as the following:
The amount of concentrated solution divided by the total volume of solution
The volume of water added- 300 ul
1. The total volume of solution will be- 100+ 300 ul= 400 ul
Df= 100/400
= 1/4 r 4 fold or 4 times
2.The volume of solution added- 100 ul
solution added to- 200ul
The total volume will be- 300 ul
Df- 100/300 or 3 times
Therefore the final dilution factor will be- 4 x 3= 12
