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aloraharding

Answer:

which off the following is a paliindromic restriction site

E GCAGCA-3

ajeigbeibraheem

The palindromic restriction site can be seen in Option D. 5' - ACGCGT - 3' from the following given options in the question.

A palindromic restriction site occurs in restriction enzymes that cut the double-stranded DNA of the enzyme at specific locations. They do so when recognized that the sequence is palindromic

The palindromic sequence is a little series of base pairs that follow a set of ordered rules.

We know that;

  • Adenine A = Thymine T
  • Guanine G = Cytosine C

So, we will pick the first three pairs of the given bases and complement them in reverse order.

Then,  reverse the order of the complement and put it in front of the end of the forward strand.

Starting with Option A

5' - ACGGCA - 3'

  • ACG → TGC
  • the reverse of the complement = CGT
  • adding it to the forward strand, we have 5' - ACGCGT - 3' which is not equal to the given option.

Option B

5' - TGCGCC - 3'

  • TGC → ACG
  • the reverse of the complement = GCA
  • adding it to the forward strand, we have 5' - TGCGCA - 3' which is not equal to the given option.

Option C

5' - AAGGTT - 3'

  • AAG → TTC
  • the reverse of the complement = CTT
  • adding it to the forward strand, we have 5' - AAGCTT - 3' which is not equal to the given option.

Option D

5' - ACGCGT - 3'

  • ACG → TGC
  • reverse of the complement = CGT
  • adding it to the forward strand, we have 5' - ACGCGT - 3' which is equal to the given option.

Option E

5' - GCAGCA - 3'

  • GCA → CGT
  • the reverse of the complement = TGC
  • adding it to the forward strand, we have 5' - GCATGC - 3' which is not equal to the given option.

Therefore, we can conclude that option D is the only correct palindromic restriction site.

Learn more about the palindromic restriction site here:

https://brainly.com/question/8886948?referrer=searchResults

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