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Which of the following correctly matches the first step of polymerase chain reaction (PCR) with its events?

a
Annealing - Taq polymerase extends the primers, synthesizing DNA
b
Evacuation- poly A segments fuse with primers to initiate sequencing
c
Denaturation - high temperature separates the DNA
d
Extension - reaction is cooled and primers bind to complementary sequences on template DNA.

The first step in a PCR cycle is the denaturation step. This is the PCR step in which the hydrogen bonds holding the complementary strands of DNA together are broken

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samueladesida43 samueladesida43 · Answer 2 · 2021-08-17T14:32:42+02:00

The first step of the polymerase chain reaction (PCR) is Denaturation, where high temperature is used to separate the DNA.

WHAT IS PCR?

PCR is an acronym for polymerase chain reaction, which is a biological technique used to amplify a DNA segment to generate several copies of it.

The polymerase chain reaction is based on the principle of thermal cycling of the DNA template.

STEPS INVOLVED IN PCR;

Polymerase chain reaction technique occurs majorly in three steps namely: denaturation, annealing and elongation.

The first step, which is DENATURATION, is when heat (about 95°C) is used to separate double-stranded DNA template into two single strands by breaking the hydrogen bonds that holds nitrogenous bases together.

Hence, the first step of the polymerase chain reaction (PCR) is Denaturation, where high temperature is used to separate the DNA.

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