Suppose a scientist identified two yeast mutants, called huh1 and huh2. The mutants have constitutive expression of the nop operon, which includes the genes nop1 and nop2. One of the mutants is a point mutation in a DNA-binding site, and the other is a deletion of the gene which encodes the DNA-binding protein.


Which term describes the regulatory function of the DNA-binding protein?


The scientist then performs a experiment mating the two haploid yeast together to determine which mutation is in the DNA-binding protein and which is in the dna-binding site. The experimental setup is listed below, wherein a minus indicates a lose-of-function mutation, and a plus indicates the wild type allele.


How will nop1 and nop2 be expressed if huh1 encodes the dna-binding protein and huh2 is a mutation in the binding site? How will nop1 and nop2 be expressed if huh2 encodes the DNA-binding protein and huh1 is a mutation in the binding site?