Professor George Palade’s elegant experiment to follow protein synthesis and trafficking, published nearly 60 years ago, provided us with a great deal of information and has been used as a tool by several investigators. If you had access to all the reagents needed to repeat the in vitro experiment, describe what you would need to do to see the progression of newly synthesized proteins and their transport in the cell.

After experiments with Guinea Pigs, Palade transitioned to anything in vitro research in which sections of pancreatic tissue had all been subjected to a pulsed radioactive lysine for something like a shorter amount of time. The radiation leucine pulse must have been subsequently adopted with nonradioactive leucine, as well as the pieces have been fixed but instead analyzed besides electron microscopy as well as transmission line during some different points in time.
Effects from either the radioactive elements with leucine throughout the reflective coating surrounding the tissue fragments formed autoradiographic particles.
Such granules eventually passed forward towards the Golgi throughout corresponding different points in time, as well as subsequently towards the cell membranes, whereby they seemed equipped for induction as established zymogen granulates.