Answer:
Major limitation of Jorge's experiment are -:
Explanation:
ABOUT VITAMINS -:
A vitamin is an organic molecule that is an important micronutrient that an organism requires in limited amounts for the proper functioning of its metabolism (or a chemically closely connected group of molecules, i.e. vitamins). In the body, basic nutrients can not be synthesized in adequate amounts, either at all or not, and must therefore be ingested via a diet.
Hence, the major limitations of the experiment are mentioned above.