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Sunnybird2006

How do the strands separate during PCR? The DNA polymerase breaks the hydrogen bonds between the two strands. The primers separate the strands during the annealing step. The high heat of the denaturation step breaks the hydrogen bonds between the two strands.

mahima6824soni

The two things that can cause a DNA helix to separate into two strands during PCR are initiation and unwinding.

What is PCR?

PCR is a polymerase chain reaction. The process is making more DNA sequences from a DNA sequence. The process is done in a PCR machine. The DNA template is put in the machine and a copy of the DNA is made.

Thus, the two things are initiation and unwinding

Learn more about PCR

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