Put the steps of chromosome spread preparation in the correct order. Think about how each step relates to the end goal of allowing you to visualize chromosomes. Note: You may need to complete additional research.

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1.Stain with Giemsa dye and observe the chromosomes under a microscope.
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2.Swell and drop cells onto microscope slides. Cells burst spreading out the chromosomes.
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3.Culture, or grow the cells in a lab, for up to two weeks.
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4.Add cell sample to the culture media, a sterile solution that helps the cells grow.
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5.Arrest, or halt, cells in metaphase. To get all the cells to this specific stage, the cells are treated with a chemical which stops cell division at that point.