Size exclusion chromatography (SEC) separates molecules based on their size by filtration through a gel.
What is size exclusion chromatography?
Size exclusion chromatography (SEC) splits molecules based on their size by filtration via a gel. The gel consists of spherical beads including pores of specific size distribution. Separation happens when molecules of various sizes exist included or excluded from the pores within the matrix.
Size exclusion chromatography exists named gel filtration chromatography because the gel effectively allows for the filtering of molecules from a sample based on molecular size. However, unlike other methods, the larger molecules elute first.
Size-exclusion chromatography (SEC), also understood as molecular sieve chromatography, exists a chromatographic procedure in which molecules in solution exist separated by their size, and in some cases molecular weight. It exists usually applied to large molecules or macro molecular complexes such as proteins and industrial polymers.
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