Answer:
Hydrolysis of pyrophosphate
Explanation:
The hydrolysis of pyrophosphate into two molecules of inorganic phosphate (Pi) reaction which is highly energetic that drives the other two reactions. These two reactions highly exergonic reactions that take place inside the aminoacyl-tRNA synthetase which is specific for that amino acid. Exergonic reactions are the reactions that produce free energy that can be used in the next phases of reactions so we can say that hydrolysis of pyrophosphate is responsible for the happening of aminoacylation reaction.
