The correct steps of the genetic engineering process include isolating DNA, disjoining the isolated DNA with restriction enzymes, inserting the desired gene, joining fragments with ligase, inserting the recombinant DNA into bacteria, and letting bacteria reproduce to clone the gene.
The genetic engineering process
The steps involved in genetically modifying organisms are as follows:
- The desired gene is first isolated
- The DNA of the target organism is also isolated
- The DNA of the target organism is disjoined with restriction enzymes
- The desired gene is inserted through the sticky ends of the disjointed DNA
- The DNA of the target organism is then joined using ligase
- The recombinant DNA is inserted into bacteria
- The bacteria are allowed to reproduce to clone the gene.
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