You want to use CRISPR/Cas9 to model a deletion mutation commonly found in cancer cells. Suppose you design two sgRNAs to this region of the genome. If Cas9 cuts DNA at 2 loci, what is the approximate size of the DNA fragment expected to be deleted

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06-06-2022
Biology

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You want to use CRISPR/Cas9 to model a deletion mutation commonly found in cancer cells. Suppose you design two sgRNAs to this region of the genome. If Cas9 cuts DNA at 2 loci, what is the approximate size of the DNA fragment expected to be deleted

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luisvaschetto luisvaschetto · Answer 1 · 2022-06-07T14:16:53+02:00

The approximate size of the DNA fragment expected to be deleted is equal to the distance between both loci (assuming linked loci).

What is CRISPR/Cas?

The CRISPR/Cas tool is a genome editing system used to cut DNA in the lab with excellent precision.

The CRISPR/Cas system can cut several loci simultaneously according to the recognition sequence.

In conclusion, the approximate size of the DNA fragment expected to be deleted is equal to the distance between both loci (assuming linked loci).

Learn more about CRISPR/Cas here:

https://brainly.com/question/22549100

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