Respuesta :
It is outlined how to conduct a spectrophotometric test to measure the rates at which oxaloacetate and l-malate enter mitochondria. The test is based on the detection of intramitochondrial NADH oxidation by oxaloacetate and intramitochondrial NAD+ reduction by malate.
The rate of interaction between the dicarboxylic acids and intramitochondrial NAD+ and NADH may be increased by between 100 and 1000 times when the mitochondrial structure is disrupted, demonstrating that the rate of entrance of both oxaloacetate and l-malate into mitochondria is restricted.
What is the Abstract of Haslam JM?
- Oxaloacetate and malate were able to enter the mitochondria of the liver, kidney, and heart at rates that were up to 50 times higher when an energy source, such as ATP or ascorbate + NNN′N′-tetramethyl-p-phenylenediamine, was added.
- Changes in intramitochondrial NAD+ and NADH concentrations caused by the addition of l-malate or oxaloacetate were followed by parallel changes in the concentrations of NADP+ and NADPH in the absence of an energy source, indicating the presence of an energy-independent transhydrogenase system in the mitochondria.
- The findings are explained in light of the theory that malate serves as a conduit for reducing equivalents between the cytoplasm and mitochondria.
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